BBB ORGAN

General

2. Number of units per plate

3. No of Plates

4. Gel volume tolerance percentage

5. Cell density tolerance percentage

7. Endothelial Cells

8. Pericytes

9. Astrocytes

Fibrinogen + Thrombin Gel for BBB

12. Volume of Fibrinogen-Thrombin-cell gel

13. Tolerance volume

14. Volume of gel mix per plate

15. Total gel for all plates

Working Fibrinogen Solution

18. Phenol red color factor

19. Required Total Fibrinogen gel working solution

20. Fibrinogen working solution concentration

mg/ml

21. Mass of phenol red to add to PBS

22. Volume of PBS with phenol red

23. Fibrinogen powder

Stock solution of Thrombin

26. Number of NIH Units

27. U/mg Solid

U/mg

28. Solid in bottle

29. Units in bottle

30. Desired concentration of Thrombin Stock Solution

U/ml

31. Desired volume of stock solution

32. Thrombin stock solution concentration in mg/ml

mg/ml

Thrombin 1X Working solution

35. Required Total thrombin gel working solution

36. Required thrombin concentration

U/ml

37. Thrombin working solution

38. Volume of heparin medium for required concentration

39. Heparin-Thrombin Solution

Cells Available from T flasks

42. Number of Endothelial cells from T-flask

cells

43. Number of flasks

44. Total Number of endothelial cells

cells

46. Number of Pericytes from T-flask

cells

47. Number of flasks

48. Total number of pericytes

cells

50. Number of Astrocytes from T-flask

cells

51. Number of flasks

52. Total number of Astrocytes

cells

Cells for BBB

55. Desired cell density for Endothelial cells in channel

cells/ul

56. Desired cell density for Pericytes in channel

cells/ul

57. Desired cell density for Astrocytes in channel

cells/ul

58. Number of Endothelial cells required for the experiment

cells

59. Number of Pericytes required for the experiment

cells

60. Number of Astrocytes required for the experiment

cells

61. Resuspension Endothelial cell density in vial

cells/ul

62. Resuspension Pericytes density in vial

cells/ul

63. Resuspension Astrocytes density in vial

cells/ul

64. Total Volume of cells for thrombin mix

65. Resuspension volume in the Endothelial cells tube

66. Resuspension volume in the Pericytes tube

67. Resuspension volume in the Astrocytes tube

68. Volume of Endothelial Resuspension for thrombin mix

69. Volume of Pericyte Resuspension for thrombin mix

70. Volume of Astrocyte Resuspension for thrombin mix

71. Volume of Endothelial cell resuspension left

72. Volume of Pericytes resuspension left

73. Volume of Astrocytes resuspension left

74. Number of Endothelial Cells remaining

cells

75. Number of Pericytes remaining

cells

76. Number of Astrocytes remaining

cells

Contact Information

Name

E-mail Id